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KMID : 0613820090190121787
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Journal of Life Science
2009 Volume.19 No. 12 p.1787 ~ p.1793
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Over-expression of NSAID Activated Gene-1 by Caffeic Acid Phenethyl Ester
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Jang Min-Jung
Kim Hyo-Eun
Son Seong-Min
Kim Min-Jeong
Seo Eul-Won
Kim Young-Ho
Kim Jong-Sik
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Abstract
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To investigate whether caffeic acid phenethyl ester (CAPE) could affect cancer cell viabilities and gene expression, human colorectal HCT116 cells were incubated with CAPE. CAPE decreased cancer cell viabilities and induced apoptosis in a dose-dependent manner. To analyse differently expressed genes by CAPE, we performed oligo DNA microarray analysis. We found that 266 genes were up-regulated more than twofold, whereas 143 genes were down-regulated more than twofold by 24 hr of treatment with 20 μM CAPE. Among the up-regulated genes, we selected 3 genes (NSAID activated gene-1 [NAG-1], cyclin-dependent kinase inhibitor 1A [CDKN1A, p21] and growth arrest and DNA-damage-inducible alpha [GADD45A]) and performed reverse-transcription PCR to confirm microarray data. In addition, we found that CAPE increased NAG-1 gene and NAG-1 protein expression in a dose-dependent manner. And, several other phytochemicals (resveratrol, genistein, daidzein and capsaicin) also could induce NAG-1 expression in human colorectal HCT116 cells. However, CAPE was the highest inducer of NAG-1, even in low concentrations. Overall, these results imply that cancer cell death by CAPE is closely related with over-expression of NAG-1.
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KEYWORD
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Phytochemical, CAPE, DNA microarray, gene expression, NAG-1
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